############### NOMAD README ####################### NOMAD, an open source system for storing and querying the results of microarray experiments. It is being developed in conjunction between three labs at the University of California, San Francisco and Lawrence Berkeley National Laboratory. NOMAD handles microarray data from the time the chip's image has been described in a text file up to the execution of complex analyses. The system is being continually broadened to encompass other partner software, but we have concentrated on its use in conjunction with Axon's GenePix and Mike Eisen's AERIE analysis engine. For installation instructions read the documentation in the doc subdirectory Please also read the "Important Notes" section below and the RELEASE_NOTES, describing important limitations of the current release To use NOMAD, use your web browser and go to the address http://your.host.here/NOMAD You will be presented with a screen prompting you to log in, you will need to create a user account. Click on the Create User link on the bottom left of the page and fill in your details as prompted. You will be taken to a page telling you that the account was created successfully, click the link to log in. Enter your username and password into the boxes provided (NB, the pasword and username are case-sensitive). Once logged in you will find youself on your NOMAD home page. From this page you can access the different parts of NOMAD and using the treeview frame on the left, browse your projects, experiments and arrays. For more detailed usage instructions please consult either the tutorial built into NOMAD, or the USAGE document in the docs subdirectory. (Note:These are incomplete and/or absent in the alpha release) ######################### Important Notes ############################## 1) Full validation of text enter as parameter names is not yet performed. Certain charcters cause problems when NOMAD tries to use them as column names in MySQL. Please avoid starting parameter names with a number or including a hyphen ('-') anywhere in the name If you do use a character that causes problems NOMAD will not warn you, or produce an error message, however the data will not be written correctly to the database. This issue is expected to be resolved in future releases by performing checks on the names of parameters and warning when a illegal character is used. 2) Number of queries while using navigation tree is limited. The current version of NOMAD can only perform qnavigation tree queries with up to 30 "Blocks", 30 Experiments and 30 Arrays. This limitation is expected to be resolved in future releases of NOMAD. 3) The spots from arrays submiited to a pre-alpha version of NOMAD will not show up in queries. This is because we changed the way spots are stored. The next release will re-enable access to these spots. 4) Please READ the information about upgrading from pre alph releases if you are doing such an update, there is critical information on the website at sourceforge. 5) Aerie must be installed as /usr/local/aerie/aerie to work with NOMAD In order to obtain the tarball, you must register first for software downloads at http://rana.lbl.gov/EisenSoftwareRegistration.htm The aerie tarball is available from http://rana.lbl.gov/downloads/aerie/ For more information see # README v1.01a Last Modified 01/08/2002